Utilisation of Adipose Derived Stem Cells from Breast Cancer Patients for Breast Regeneration Post-Mastectomy
Association of Breast Surgery ePoster Library. O' Halloran N. 05/15/17; 166205; P002
Dr. Niamh O' Halloran
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Abstract
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Introduction
Current breast reconstruction methods, namely implant and autologous reconstruction, are complicated by events such as capsular contracture, donor site morbidity and flap necrosis. Adipose derived stem cells (ADSCs) are becoming the gold standard for adipose regeneration, and may potentially be utilised for breast reconstruction post-mastectomy.[1] Multipotent ADSCs can be isolated from patients' own adipose tissue and cultured ex vivo for reimplantation. The aim of this study was to analyse the suitability of utilising ADSCs isolated from cancer patients for adipose regeneration.
Methods
ADSCs were harvested from breast cancer patients who have and have not had chemotherapy at the time of tumour excision and from healthy controls and were cultured in vitro. Cell morphology of each group was examined and Oil Red O staining confirmed ADSC adipogenic potential after treatment with induction media.
Results
ADSCs were isolated and cultured from 15 patients: 5 breast cancer patients with neoadjuvant chemotherapy; 5 breast cancer without neoadjuvant chemotherapy; and 5 healthy controls. ADSCs isolated from breast cancer patients possess similar cell surface markers as those from healthy controls by immunophenotyping e.g. CD31, CD34, CD45, CD73, CD90 and CD105. There were no significant differences in cell morphology. Adipose induction was successful in all 3 groups and quantified by photospectrometry.
Conclusion
Adipose tissue from breast cancer patients, irrespective of receipt of neoadjuvant chemotherapy is a potential source of ADSCs for breast regeneration.
1. Combellack, E.J., et al., Adipose regeneration and implications for breast reconstruction: update and the future. Gland surgery, 2016. 5(2): p. 227.
Current breast reconstruction methods, namely implant and autologous reconstruction, are complicated by events such as capsular contracture, donor site morbidity and flap necrosis. Adipose derived stem cells (ADSCs) are becoming the gold standard for adipose regeneration, and may potentially be utilised for breast reconstruction post-mastectomy.[1] Multipotent ADSCs can be isolated from patients' own adipose tissue and cultured ex vivo for reimplantation. The aim of this study was to analyse the suitability of utilising ADSCs isolated from cancer patients for adipose regeneration.
Methods
ADSCs were harvested from breast cancer patients who have and have not had chemotherapy at the time of tumour excision and from healthy controls and were cultured in vitro. Cell morphology of each group was examined and Oil Red O staining confirmed ADSC adipogenic potential after treatment with induction media.
Results
ADSCs were isolated and cultured from 15 patients: 5 breast cancer patients with neoadjuvant chemotherapy; 5 breast cancer without neoadjuvant chemotherapy; and 5 healthy controls. ADSCs isolated from breast cancer patients possess similar cell surface markers as those from healthy controls by immunophenotyping e.g. CD31, CD34, CD45, CD73, CD90 and CD105. There were no significant differences in cell morphology. Adipose induction was successful in all 3 groups and quantified by photospectrometry.
Conclusion
Adipose tissue from breast cancer patients, irrespective of receipt of neoadjuvant chemotherapy is a potential source of ADSCs for breast regeneration.
1. Combellack, E.J., et al., Adipose regeneration and implications for breast reconstruction: update and the future. Gland surgery, 2016. 5(2): p. 227.
Introduction
Current breast reconstruction methods, namely implant and autologous reconstruction, are complicated by events such as capsular contracture, donor site morbidity and flap necrosis. Adipose derived stem cells (ADSCs) are becoming the gold standard for adipose regeneration, and may potentially be utilised for breast reconstruction post-mastectomy.[1] Multipotent ADSCs can be isolated from patients' own adipose tissue and cultured ex vivo for reimplantation. The aim of this study was to analyse the suitability of utilising ADSCs isolated from cancer patients for adipose regeneration.
Methods
ADSCs were harvested from breast cancer patients who have and have not had chemotherapy at the time of tumour excision and from healthy controls and were cultured in vitro. Cell morphology of each group was examined and Oil Red O staining confirmed ADSC adipogenic potential after treatment with induction media.
Results
ADSCs were isolated and cultured from 15 patients: 5 breast cancer patients with neoadjuvant chemotherapy; 5 breast cancer without neoadjuvant chemotherapy; and 5 healthy controls. ADSCs isolated from breast cancer patients possess similar cell surface markers as those from healthy controls by immunophenotyping e.g. CD31, CD34, CD45, CD73, CD90 and CD105. There were no significant differences in cell morphology. Adipose induction was successful in all 3 groups and quantified by photospectrometry.
Conclusion
Adipose tissue from breast cancer patients, irrespective of receipt of neoadjuvant chemotherapy is a potential source of ADSCs for breast regeneration.
1. Combellack, E.J., et al., Adipose regeneration and implications for breast reconstruction: update and the future. Gland surgery, 2016. 5(2): p. 227.
Current breast reconstruction methods, namely implant and autologous reconstruction, are complicated by events such as capsular contracture, donor site morbidity and flap necrosis. Adipose derived stem cells (ADSCs) are becoming the gold standard for adipose regeneration, and may potentially be utilised for breast reconstruction post-mastectomy.[1] Multipotent ADSCs can be isolated from patients' own adipose tissue and cultured ex vivo for reimplantation. The aim of this study was to analyse the suitability of utilising ADSCs isolated from cancer patients for adipose regeneration.
Methods
ADSCs were harvested from breast cancer patients who have and have not had chemotherapy at the time of tumour excision and from healthy controls and were cultured in vitro. Cell morphology of each group was examined and Oil Red O staining confirmed ADSC adipogenic potential after treatment with induction media.
Results
ADSCs were isolated and cultured from 15 patients: 5 breast cancer patients with neoadjuvant chemotherapy; 5 breast cancer without neoadjuvant chemotherapy; and 5 healthy controls. ADSCs isolated from breast cancer patients possess similar cell surface markers as those from healthy controls by immunophenotyping e.g. CD31, CD34, CD45, CD73, CD90 and CD105. There were no significant differences in cell morphology. Adipose induction was successful in all 3 groups and quantified by photospectrometry.
Conclusion
Adipose tissue from breast cancer patients, irrespective of receipt of neoadjuvant chemotherapy is a potential source of ADSCs for breast regeneration.
1. Combellack, E.J., et al., Adipose regeneration and implications for breast reconstruction: update and the future. Gland surgery, 2016. 5(2): p. 227.
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